Abstract:

A HPLC-ELSD method was established for the simultaneous quantitative analysis of glucose, fructose, sucrose, GF2, GF3, and GF4. The chromatographic separation was achieved on an amino column using a mobile phase composed of a mixture of acetonitrile and water at a flow rate of 1.0 ml/min by gradient elution. The ELSD drift tube temperature was set at 85 ℃ and the carrying gas was air. Under the above conditions, all glucose, fructose, sucrose, GF2, GF3 and GF4 could be well separated, and the spiked recoveries were 97.62%–99.55% with a RSD less than 3% (n = 5). This method is accurate, rapid, simple and reproducible, and thus it is suitable for the determination of monosaccharides, disaccharides, and oligosaccharides.

Key words: HPLC-ELSD, monosaccharide, disaccharide, fructo-oligosaccharides